TRANSEPITHELIAL POTENTIAL AND ALKALIZATION IN AN IN SITU PREPARATION OF TOBACCO HORNWORM (MANDUCA SEXTA) MIDGUT
نویسندگان
چکیده
منابع مشابه
Transepithelial Potential and Alkalization in an in Situ Preparation of Tobacco Hornworm (manduca Sexta) Midgut
The midgut of larval lepidopteran insects rapidly raises the pH of the entering food from approximately neutral values to as high as pH 12 in some species (Waterhouse, 1949; Dow, 1984). These values are among the highest achieved by biological systems and are all the more remarkable in view of the high rate of food consumption relative to body mass in lepidopteran larvae. The isolated midgut of...
متن کاملExcretory role of the midgut in larvae of the tobacco hornworm, Manduca sexta (L.).
Caterpillars of Manduca sexta use two distinct transport mechanisms for the excretion of dyes. One pump (Type A) has a high affinity for acid (anionic) dyes and occurs in the midgut and medial Malpighian tubules. Acid dyes accumulate rapidly in the lumen of the midgut while the Malpighian tubules appear to play only a minor role in the excretion of these dyes. The other pump (Type B) excretes b...
متن کاملInhibition of midgut ion transport by allatotropin (Mas-AT) and Manduca FLRFamides in the tobacco hornworm Manduca sexta.
Short-circuit current (Isc) across the posterior midgut of day 2 fifth-instar tobacco hornworms (Manduca sexta) is inhibited by Manduca allatotropin (Mas-AT) and two Manduca FLRFamides (F7G and F7D). Another FLRFamide, F10, and the related molluscan tetrapeptide FMRFamide elicited only a modest inhibition of Isc. Mas-AT, F7G and F7D inhibited the Isc in a dose-dependent manner. Maximal inhibiti...
متن کاملSex-Specific Odorant Receptors of the Tobacco Hornworm Manduca Sexta
As odor information plays a vital role in the life of moths, their olfactory sense has evolved into a highly specific and sensitive apparatus relevant to reproduction and survival. The key players in the detection of odorants are olfactory receptor (OR) proteins. Here we identify four OR-encoding genes differentially expressed in the antennae of males and females of the sphingid moth Manduca se...
متن کاملAn Investigation of the Midgut K+ Pump of the Tobacco Hornworm (manduca Sexta) Using Specific Inhibitors and Amphotericin B
Active K+ secretion in isolated posterior midguts of Manduca sexta was studied by measuring the short-circuit current. One aim of this study was to verify the postulate from biochemical reports that the cooperative apical arrangement of a vacuolar-type H+-ATPase (V-ATPase) and a K+/H+ antiporter drive the short-circuit current. Hence, we tested several specific inhibitors of the V-ATPase on the...
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ژورنال
عنوان ژورنال: Journal of Experimental Biology
سال: 1994
ISSN: 1477-9145,0022-0949
DOI: 10.1242/jeb.194.1.341